Detection of Virulence Genes and Biofilm Forming Capacity of Diarrheagenic E. coli Isolated from Different Water Sources

Diarrheagenic Escherichia coli (DEC) are associated with frequent incidences of waterborne infections and pose health risk to individuals who contact water for domestic or recreational uses. Detection DEC pathotypes in drinking can be used as an indicator fecal contamination. This study aimed investigate the occurrence their capacity form biofilms samples collected from different sources. In this study, PCR analysis was determine four clinically significant virulence genes diarrheagenic E. coli, eaeA (Enteropathogenic coli), stx1, stx2 (Enterohemorrhagic coli) sth (Enterotoxigenic (n = 35) by using specific primers conditions. amplicons were visualized agarose gel electrophoresis. A total 12/35 (34%) detected positive at least one 11/12 (91%) isolates harbored gene while 1/12 (8%) none. The most (75%), stx1 (66%). Biofilm assay confirmed that ETEC cause damage enteric walls attaching effacing persist diarrheal indicated sources is contaminated potential it a source diseases. amplification (EPEC, EHEC ETEC) demonstrates potentially virulent distributed may concern. There is, therefore, urgent need monitor water.